Journal: Journal of visualized experiments : JoVE

Article Title: 4D Light-sheet Imaging of Zebrafish Cardiac Contraction

doi: 10.3791/66263

Figure Lengend Snippet: Table of Materials

Article Snippet: RESOURCE SOURCE/Reference IDENTIFIER Animal models Tg(myl7:nucGFP) transgenic zebrafish Burns Lab in Boston Children’s Hospital ZDB-TGCONSTRCT-070117-49 Software and algorithms MATLAB The MathWorks Inc. R2023a LabVIEW National Instruments Corporation 2017 SP1 HCImage Live Hamamatsu Photonics 4.6.1.2 Python The Python Software Foundation 3.9.0 Fiji-ImageJ Schneider et al. 18 1.54f 3DeeCellTracker Chentao Wen et al. 15 v0.5.2 Unity Unity Software Inc. 2020.3.2f1 Amira Thermo Fisher Scientific 2021.2 3D Slicer Andriy Fedorov et al. 17 5.2.1 ITK SNAP Paul A Yushkevich et al. 16 4 Light-sheet system Cylindrical lens Thorlabs ACY254-050-A 4X Illumination objective Nikon MRH00045 20X Detection objective Olympus 1-U2M585 sCMOS camera Hamamatsu C13440-20CU Motorized XYZ stage Thorlabs PT3/M-Z8 Two-axis tilt stage Thorlabs GN2/M Rotation stepper motor Pololu 1474 Fluorescent beads Spherotech FP-0556-2 473nm DPSS Laser Laserglow R471003GX 532nm DPSS laser Laserglow R531003FX Microinjector and vacuum pump Microinjector WPI PV850 Vacuum pump Welch 2522B-01 Pre-Pulled Glass Pipettes WPI TIP10LT Capillary tip for gel loading Bio-Rad 2239912 Virtual reality hardware VR headset Meta Quest 2 30mg/L PTU solution PTU Sigma-Aldrich P7629 1X E3 working solution - - 1% Agarose Low-melt agarose Thermo Fisher 16520050 Deionized water - - 10g/L Tricaine stock solution Tricaine Syndel SYNC-M-GR-US02 Deionized water - - Sodium bicarbonate Sigma-Aldrich S6014 150mg/L Tricaine working solution 10g/L Tricaine stock solution - - Deionized water - - 60X E3 stock solution Lab Animal Resource Center (LARC), The University of Texas at Dallas Sodium Chloride NaCl Potassium Chloride KCL Calcium Chloride Dihydrate CaCL 2 x 2H 2 O Magnesium Sulfate Heptahydrate MgSO 4 x 7H 2 O RO Water - 1X E3 working solution 60X E3 stock solution - RO Water - 1% Methylene Blue (optional) C 16 H 18 ClN 3 S Open in a separate window Table of Materials.

Techniques: Transgenic Assay, Software

Journal: Journal of visualized experiments : JoVE

Article Title: In vivo targeting of xenografted human cancer cells by functionalized fluorescent silica nanoparticles in zebrafish

doi: 10.3791/61187

Figure Lengend Snippet: Table of Materials

Article Snippet: × 0,78 mm PBS (Dulbecco’s Phosphate-Buffered Salt Solution 1X ) Corning 21–030-CV sold by Fisher Tricaine methanesulfonate Western Chemicals NC0872873 sold by Fisher DMEM (Dulbecco’s Modified Eagle’s Medium) Corning 10–013-CV sold by Fisher Trypsin-EDTA Corning MT25053CI sold by Fisher Fetal Bovine Serum Sigma-Aldrich F0926 Microloader tip Eppendorf E5242956003 sold by Fisher Magnetic stand World Precision Instruments M10 Needle Puller Sutter instruments P-97 Micromanipulator Applied Scientific Instrumentation MMPI-3 Pneumatic pico pump World Precision Instruments SYSPV820 Hemocytometer Fishersci brand 02–671–51B Plastic pipette Fishersci brand 50–998–100 Petri dish Corning SB93102 sold by Fisher Tweezer Fishersci brand 12–000–122 Razor blade Fishersci brand 12–640 P200 tip Fishersci brand 07–200–293 Olympus MVX-10 fluorescent microscope Olympus MVX-10 SZ51 dissection microscope Olympus SZ51 Fish incubator VWR 35960–056 Computer and monitor ThinkCentre X000335 Open in a separate window Table of Materials

Techniques: Western Blot, Modification, Transferring, Microscopy, Dissection